Specific pathogen Free

Research advance in the specific pathogen free swine

关于无特定病原猪的研究进展

A Study on Methods of Rearing and Repopulation With Specific Pathogen Free Stock in Swine Industry of China

中国养猪业的无特定病原（SPF）猪群的培育和推广方法的研究

Preparation of Influenza A Virus Antiserum from Chickens with Specific Pathogen Free

用SPF莱亨鸡制备抗甲型流感病毒血清

Molecular Analysis of Intestinal Microflora of SPF ( Specific Pathogen Free ) Piglets

SPF仔猪肠道菌群结构的分子分析

The Facility Establishment of the Specific Pathogen Free Laboratory Animal Centre of Guangxi Institute for Drug Control

广西药品检验所SPF级实验动物中心设施的建立

The fifteen newborn SPF ( Specific Pathogen Free ) piglets obtained by caesarean operation were randomly divided into three groups .

通过无菌剖腹产获取15头无特定病原菌（SpecificPathogenFree，SPF）仔猪，随机分为三组。

This paper summarizes the history , use significance , breeding , management and determination method of the specific pathogen free swine ( SPF swine ) .

本文就无特定病原（SPF）猪的历史，使用SPF猪的目的意义，SPF猪的培育和管理以及SPF猪的鉴定方法等进行了综述。

Methods Spleen cells of BALB / c mice raised in specific pathogen free conditions were directly fused with Sp2 / 0 myeloma cells .

方法取饲养在无特殊病原体条件下BALB/c小鼠的脾细胞，直接与SP2/0细胞融合。

MATERIALS : Twenty-four specific pathogen free ( SPF ) unmated SD rats were selected , aging 4 and half months and weighing 230 ± 15 g.

对象：清洁级4个半月龄未交配的雌性SD大鼠24只，体质量（230±15）g。

Using SPF ( Specific pathogen free ) animals as the breeding stock and adopting synthesize preventive measure , the experiment mouse and rat of clean grade were reared in barrier system for 2 years .

以SPF级（无特殊病原体）动物为种源，采取综合防治措施，在屏障设施饲养清洁级实验小鼠和大鼠。

Metho-ds : Healthy , adult chickens with specific pathogen free ( SPF ) were immunized with two different H11-subtype influenza A virus strains and stimulated to produce antiserum .

方法：用两株H11亚型流感病毒分别免疫成年、健康的SPF莱亨鸡，刺激其产生抗体。

According to the disparities of carrying microorganisms and parasites , laboratory animals can be classified into four grades : normal animal , clean animal , specific pathogen free animal ( SPF animal ) and sterile animal .

实验动物按其微生物、寄生虫控制程度，可划分为四个等级，即普通动物、清洁动物、无特定病原体动物（SPF动物）和无菌动物。

Only 71 research papers used clean animal and specific pathogen free animal ( SPF ) as laboratory animals and 49 research papers mentioned the application conditions of the animals experiments , which occupied 15 57 % and 10 45 % of the totals .

只有71篇论著使用的实验动物是清洁级或SPF级，49篇论著提及了实验动物的应用环境，分别占有关论文总数的1557%和1045%；

MATERIALS : Twenty specific pathogen free ( SPF ) normal female BALB / c nude mice weighing ( 10 ± 2 ) g , aged 3 to 4 weeks , were purchased from the Experimental Animal Center of the Third Military Medical University .

材料：20只健康雌性BALB/c裸小鼠，SPF级，体质量（10±2）g，鼠龄3～4周，购自第三军医大学实验动物中心。

Livers , spleens and intestines obtained from died poults were homogenized and centrifuged . The supernatant was inoculated into 15-day-old specific pathogen free ( SPF ) embryonated chicken eggs to propagate the viruses .

取病死火鸡肝、脾、肠匀浆，取上清液通过尿囊腔接种15日龄SPF鸡胚。

SETTING and PARTICIPANTS : The experiment was completed in the Department of Protective Medicine , Institute of Naval Medicine , using specific pathogen free ( SPF ) male BALB / c nude mice ( 6 weeks old ) purchased from Experimental Animal Laboratory of Shanghai Institute of Oncology .

地点和对象：在海军医学研究所防护医学研究室完成。雄性BALB/c裸鼠，6周龄，SPF级，购自上海市肿瘤研究所实验动物研究室。

Six virus strains were isolated from affected chickens in some areas of Shandong province , and characterized as Newcastle disease virus by hemagglutination / hemagglutination_inhibition test , challenge with 6_week specific pathogen free ( SPF ) chickens and inoculation of embryos after neutralization using particular sera .

从山东省不同地区的发病鸡群中分离了6株病毒，经HA、HI试验、病毒回归试验和血清中和接种鸡胚试验确定所分离的毒株为新城疫病毒。